No. Distinguish capital actually committed to the enterprise from personal reserves and uncommitted balances. Savings available for future spending do not all become investment merely by appearing in the application’s financial records.
Prepare a use-of-funds schedule
Show commercial payments, retained operating funds and personal reserves clearly, with review of which amounts qualify. Allow separately for current government charges, professional preparation, translations and travel. Selling investments can also involve transaction costs that explain the net proceeds; do not confuse those costs with money received by the enterprise.
There is no universal savings amount that guarantees E-2 eligibility. Prepare a use-of-funds schedule rather than presenting a balance, since the two answer different questions and only one of them is useful. The schedule should show what each amount is for: capital committed to the enterprise, working capital to fund it until revenue arrives, household relocation and living costs, and reserves retained for contingencies.
Listing an entire savings balance as investment overstates the commitment and understates the household's own requirements, and it tends to fall apart when someone asks how the family intends to live. Keep the personal funds visibly outside the business schedule. Then evidence each business line with the payment or commitment behind it.
Immigration-related charges are separate again and vary with the procedure and the number of family applicants, so verify them against current official information.